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The cell precipitate was resuspended in Neurobasal medium (Gibco, 21103049) supplemented with 2% B-27 (Gibco, 17504044) and 0.5 mM l-glutamine, and the cells were seeded on plates pre-coated with poly-d-lysine hydrobromide (Sigma-Aldrich, P0296)

In addition, earlier studies have shown that the interaction of rutin with ascorbic acid leads to an increase in the reducing properties of ascorbic acid, which is expressed by a decrease in xanthine oxidase activity, responsible for the cytosol production of superoxide anion [74]

Transitioning from preclinical to human trials is a major step in drug development, and healthy volunteers play a key role in ensuring that investigational drugs meet safety standards before they are tested in patients