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qRT-PCR Total RNA was extracted using a HiPure Bacterial RNA Kit (Guangzhou Meiji Biotechnology Co., Ltd., Guangzhou, China) and quantified by the ratio of absorbance (260 nm/280 nm) using a Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)

However, since all the pump flow is combined into a single common pipe, your risk of overflowing a condenser is even greater than it is in a header supply and return arrangement