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Briefly, blood was collected and serum was obtained by centrifugation at 3500 rpm for 15 min at 4

To confirm this, we treated HUVECs with the proteasome inhibitor MG132 and the protein synthesis inhibitor cycloheximide (CHX, 10 g/mL)

The concentration of Human MUC1 in the samples is then determined by comparing the OD of the samples to the standard curve

While it is healthy to pass gas, excessive or smelly flatulence could indicate a potential digestive problem