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S.typhimurium Ames tester strains have rfa mutations that allow for better permeability of the outer membrane, which was examined for sensitivity to crystal violet (CASRN 54862-9, MW = 408) [19]

Using Alcian blue staining, we observed epithelial cells lining the lumen stained blue, indicating the presence of mucopolysaccharides, i.e., mucin-secreting cells (Fig

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