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On the other hand, a number of studies suggest that in pharmacologic doses (10 mM), ascorbate exhibits anti-cancer effects and may have a potential for use in the treatment of cancer through the induction of both oxidative stress and DNA demethylation [160,161]

Detection of intracellular ROS and mitochondrial membrane potential in vitro Fluorescent probes 2,7-Dichlorodihydrofluoresce in diacetate (Solarbio, China) were used to detect intracellular ROS in cells

12 mg/ml

P: 500 ug

Similarly, overexpression of SEPHS2 in METTL5-knockdown MM cells did not achieve the same overexpression efficiency at the protein level as mRNA (Supplementary Fig