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Statistical analysis Data were analyzed with SPSS Statistics 20 and GraphPad Prism 6.0 and presented as mean s.e.m or mean S.D

This barrier, however, breaks down with age and further disruption is a hallmark of many age-related disorders

Thus, methodological details such as tubes, anticoagulants, centrifugation, and delays in processing are likely sources of type 2 error in the ME/CFS cytokine literature and limit the comparability across studies