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Nevertheless, nhr-49 is needed to activate the expression of the SKN-1c isoform, the key isoform driving antioxidant responses, in a worm strain mutant for brap-2/BRCA1 Associated Protein homolog , and is essential for increased SKN-1 activity in the amdh-1/ aminohydrolase domain containing protein mutant, although it is not clear if this is a transcriptional or posttranscriptional role of NHR-49 (Hu et al., 2018

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To confirm the binding affinities of the two bispecific nanobody constructs, we also performed surface plasmon resonance (SPR) measurements to determine kD value (Fig