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Availability: InStock
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Description

Human fibroblasts listed in Supplemental Table 2 were cultured in DMEM with 10% FBS plus penicillin and streptomycin

Western blotting Cells were lysed in lysis buffer (20 mM Tris, 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA, 1 mM EGTA, 10% glycerol, and protease and phosphatase inhibitors), incubated on ice for 15 min, and centrifuged at 16,000 x g for 10 min at 4 C