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Drp1-associated mitochondrial fission has been shown to promote the formation of inflammatory vesicles and pro-inflammatory factors (e.g., cysteine-3 and IL-18), accelerating the progression of ischemic heart disease

For apoptosis induction, Jurkat cells (20,000 cells per well) were incubated with soluble human Fas ligand (30 ng ml 1 , ALX-522-020, Enzo Life Sciences) for 24 h, and Pfa1 cells (1,500 cells per well) were co-incubated with mouse TNF (10 ng ml 1 ) and BV-6 (400 nM) for 24 h

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If you miss a dose, ask your clinician how to proceed rather than doubling the next dose

Electrophysiological in vivo data analysis For the presence of seizures, data were analyzed in Spike2 (Cambridge Electronic Design Limited, UK) for visual inspection and report presence of epileptic events