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The PVDF membrane was then washed and incubated with secondary antibody (Abclonal, China, 1:2000 dilution) for 1 h at room temperature and immunoreactive bands visualized with a luminescent image analyzer and quantified by Image J 1.52a (Tanon 5200, China)

There is known familial association, with the risk being 0.7% amongst first-degree relatives and approximately 1.5% amongst siblings, both 100-times higher than that in the general population

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