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After 3 washes with TBST, the membranes were incubated in the dark with IR680- or IR800-conjugated secondary antibodies (LI-COR) at 1:10,000 dilution in 5% BSA in TBST at RT for 1 h

When single amino acids or specific combinations dominate dietary intake, competitive inhibition occurs at absorption sites, potentially creating deficiencies in other essential amino acids crucial for keratin synthesis

Results Timeline Mild dark spots often go away within 6-8 weeks of use, while stubborn marks take 2-4 months to heal

Even after receiving adequate treatment, melasma may relapse on exposure to the summer sun (particularly in Australia) or because of changes in hormonal or endogenous factors

Amplifications were performed on a CFX384 Real-Time PCR System (Bio-Rad, Hercules, CA)