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Notably, intravenous administration of 0.5 mg/kg PBGD mRNA formulated by lipid nanoparticles into Cynomolgus macaque achieved 40% and 90% increases in the activity and expression level of PBGD, respectively, in comparison to the phosphate buffered solution (PBS) treated animals [154]

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After blocking endogenous peroxidases with a blocking solution (#A600332-0100, Sangon Biotech, Shanghai, China) for 30 min, the sections were incubated overnight at 4 C in a humidified chamber with primary antibodies against RASA4 (1:100 dilution, #ab157190, Abcam, Cambridge, MA, USA) and SERPINE2 (1:100 dilution, #ab154591, Abcam, Cambridge, MA, USA)

Protein kinase C and protein kinase C play opposite roles in the proliferation and apoptosis of glioma cells