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Description

The cells were stained in FACS buffer (PBS containing 0.5% bovine serum albumin and 0.05% sodium azide) with fluorescently labelled antibodies from BioLegend (CD11c-APC/Cy7, CD11b-BV421, CD40-FITC, CD80-APC, CD86-PE, CD8-PE/Cy7, CD4-PerCp/Cy5.5, CD69-PE, CD25-APC, CD3-BV421, CD44-BD427, CD45.1-FITC, CD3-PerCp/Cy5.5, and CD44-FITC) or Tonbo Biosciences (HLA-DR-PerCp/Cy5.5) to identify DC and T cells