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Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

Due to limited long-term human safety data, most research protocols suggest intermittent administration (23 times per week) rather than daily use

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Common side effects include pain, redness, or swelling at the injection site, which typically resolve within 2448 hours

(2020) 39 ME/CFS (CDC-1994 and/or CCC criteria) were compared to 41 healthy controls, 12 patients had a very high level, while 3 had very low levels of IGHV3-23/30 protein