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doi: 10.3389/fendo.2023.1017886 Received 12 August 2022 Accepted 04 January 2023 Published 18 January 2023 Volume 14 - 2023 Edited by Surasak Saokaew, University of Phayao, Thailand Reviewed by Gholamreza Hamidian, University of Tabriz, Iran

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The culture was split into two 300 ml cultures and each flask was diluted to 1.0 10 6 cells/mL, usually leading to a final volume of 600 mL that complies with the ratio of air to cell medium of 4:1

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