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For antigen specific T cell staining, 200 ng of an MHC class I molecule fused to a Twin-Strep-tag (50 kDa) refolded with a cytomegalovirus (CMV)-derived peptide was pre-incubated with 75 ng of Strep-Tactin PE (A) or Strep-TactinXT PE (B) for 15 min at 4 C

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Phosphatase PP2A is requisite for the function of regulatory T cells

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