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Association of cerebral spinal fluid copper imbalance in amyotrophic lateral sclerosis

Another explanation could be that these adducts may break into lactone and the substrates (for example, secondary amines) of former Micheal-type addition reaction, due to the reversibility of this type of reaction (Lawrence et al., 2001)

doi: 10.1002/alz.13844, 214

The slides were washed in water and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer