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Evidence for reductive activation of the oxy-ferrous complex by tetrahydrobiopterin

After 6 days, M1 macrophages were activated using 10ng/ml human interferon and 100ng/ml LPS (Invivogen, San Diego, Calif.) and M2-like macrophages were distributed into two separate cultures, each of which was further induced into M2c macrophages by the addition of IL-10 and M2d macrophages by the addition of IL-4, IL-10 and TGF- , respectively