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NAPstar3b was found to have a slightly decreased K d(NADPH) but an approximately 3-fold higher K d(NADH) ( Figure 1CH )

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In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)

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Alantolactone and isoalantolactone prevent amyloid 25-35 -induced toxicity in mouse cortical neurons and scopolamine-induced cognitive impairment in mice