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For glutamine tracing, 4 mM of U- 13 C-glutamine was added to the glutamine-free RPMI-1640 medium with 10% v/v HI-FBS, 1 mM HEPES, and 1 mM sodium pyruvate

Emerging therapeutic strategies target specific molecular mechanisms, including PPAR- pathway modulation, BAT activation through UCP1 targeting, AMPK pathway interventions, senolytic approaches to eliminate dysfunctional adipose cells, and precise genomic and RNA-based interventions

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