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Treatment with 5FU resulted in the reorganization of mitochondrial networks into filamentous structures (Fig

L-cystine is reduced in the BBB, and the resulting cysteine is transported out of the endothelial cells into the brain via L-type amino acid transporter 1 (LAT1) (Snchez del Pino et al., 1995)

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Cells were incubated with a mouse monoclonal antibody targeting the human transferrin receptor (Clone H68.4, #136800, Invitrogen, Eugene, OR) diluted to a final concentration of 2 g/mL in PBS overnight at 4 C