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High-fat diet enhances stemness and tumorigenicity of intestinal progenitors

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A volume of 5 l of the protein-dye mixture was dispensed into a 384-well microplate (Thermo Scientific, AB1384K), followed by addition of 5 l of C1-27 and 3 l of silicone oil (to avoid evaporation)

However, as a respond, breast cancer cells increased the expression of Nrf2 that led to the activation of ARE and increased transcription of genes associated with anti-oxidation and multidrug resistance transporters, thus increasing the survival from oxidative damage induced by tamoxifen [109]

Many of these products are manufactured without strict quality controls, leading to issues with purity issues, heavy metal contamination, or the presence of residual TFA (trifluoroacetic acid) from the synthesis process