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To confirm the results, Hu et al

Then, the cells were washed with PBS three times, dried upside down, and the colonies images were finally recorded and analyzed by ImageJ ( 2.5 Cellular iron assay The FerroOrange staining assay was used to detect the content of intracellular ions (Fe 2+ ) using a reported method ( 2.6 Cell apoptosis measurements An Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis kit (Thermo Fisher Scientific, Waltham, MA, United States) was used to detect the percentage of cell apoptosis

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Age spots, melasma, or other form of hyperpigmentation can potentially be lightened with kojic acid

Sigma) supplemented with 10% fetal bovine serum (FBS