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The samples were centrifuged at 15,000 rpm for 2 min at 4 C using an Eppendorf 5424R centrifuge (Eppendorf SE, Hamburg, Germany), and the resulting supernatant was subjected to HPLC-MS/MS analysis

Parkinson's Progression Marker Initiative* (2018) Longitudinal Change of Clinical and Biological Measures in Early Parkinson's Disease: Parkinson's Progression Markers Initiative Cohort