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Each biopsy was fixed in 10% formalin, routinely processed, embedded in a paraffin block, sectioned by the ordinary microtome into 5 m thick sections and stained with hematoxylin and eosin (H and E) and Masson fontana (MF) for detection of melanin

Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95