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CRL-2266, New York, NY, USA) and was grown in MEM:F12 media (1:1) with 15% FBS, 100 U/ml penicillin, and streptomycin at 37 in a humidified 5% CO 2 incubator

Requires 200 1000 g lysate starting material per assay The Rho-GTP binding domain (RBD) of the human Rhotekin protein has been overexpressed as a GST-tagged recombinant protein in a bacterial expression system and bound to colored glutathione Sepharose beads

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This is the most common technique error we see in self-administration protocols

doi: 10.1016/j.fitote.2016.02.007