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After transfection for 72 h, the nuclei were stained with DAPI (Beyotime), and the immunofluorescence of cells was observed using a fluorescence microscope

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The highly selective permeability of the BBB largely depends on the presence of continuous TJs that connect ECs, and so it follows that dysregulation of the expression of TJ proteins, such as occludin, claudin-5 and ZO-1, can critically influence BBB permeability

This is especially important for patients who will be getting regional anesthesia, such as a spinal block or epidural