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10.1172/JCI176660 39 ZhangC.XieY.ChenH.LvL.YaoJ.ZhangM.et al (2020)

adjust to 6.0 to 6.5 with sodium hydroxide solution if needed

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Numerous studies have documented a reduction in NAD + levels across various mouse tissues, including adipose (Yoshino et al., 2011), skeletal muscle (Frederick et al., 2015), liver (Trammell et al., 2016), and hypothalamus (Sasaki et al., 2014), concomitant with the development of obesity