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Yes , for any in vitro biological assay where the reconstituted peptide is added to cell cultures or tissue preparations, matched Bacteriostatic Water vehicle controls at the same final benzyl alcohol concentration as the highest peptide treatment concentration should be included to confirm absence of benzyl alcohol-mediated biological effects on the assay endpoints measured

Is reconstitution the same as dosing

Peptide solutions, once reconstituted, have a limited shelf life

* Correspondence: Remigiusz Domin, [email protected] These authors have contributed equally to this work and share senior authorship Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

The consequences of NNMT inhibition over months or years are unknown