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NNMT Expression and Gene Structure NNMT is a cytosolic enzyme that was first purified from rat liver in 1951 ( NNMT gene was cloned and characterized by PCR, which contributed to revealing the possible molecular genetic mechanism of NNMT regulation ( NNMT gene is located on human chromosome 11q23.1 and mouse chromosome 9, and contains three exons and two introns ( NNMT gene is ~55.5-kb long and contains two major transcription initiation sites (TISs): TIS201 and TIS203