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Slides were incubated at 4 C overnight in the following primary antibodies: anti-KCC2 (1:500, Millipore Sigma C2366), anti-cFOS (1:1000, Synaptic System 226009, RRID: AB_2943525)

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Lactation is characterized by extensive morphological and metabolic changes in different tissues to guarantee a sufficient supply of blood and nutrients to the mammary gland for efficient milk production [56]

Next a small, thin plastic tube called a catheter is inserted directly into your vein in a quick split-second process which is no more than a slight pinch at worst and feels like nothing for many people

Uncontrolled injections, however, can result in renal problems, allergic reactions, or infections