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Membranes were blocked with 5% BSA in PBS-Tween-20 for 1 h at room temperature and incubated overnight at 4 C with one of the following primary antibodies: rabbit polyclonal anti-BAX (Cell Signaling Technology), rabbit polyclonal anti-BNIP3 (Cell Signaling Technology), or mouse monoclonal anti-FLAG (clone M2)

Meanwhile, the inflammatory cytokines IL-18 and IL-1 may serve as the markers of the severity of AP patients (Janiak et al., 2015)

PMID: 40500343 Cell Death Discov NDUFB7 mutations cause brain neuronal defects, lactic acidosis, and mitochondrial dysfunction in humans and zebrafish

Charging: Only use lithium-ion battery-specific chargers

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