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The cells were resuspended in lysis buffer (50 mM HEPES-NaOH pH 8.0, 300 mM NaCl, 10% glycerol, 1 mM MgCl 2 , 5 mM -mercaptoethanol 4 g/ml Avidin, 500 U Pierce Universal nuclease for cell lysis, 1 mM AeBSF, 1 Roche cOmplete EDTA-free protease inhibitor tablet per 100 mL of lysis buffer), lysed by sonication and clarified by centrifugation at ~ 35,000 x g for 45 min

Recent research has shown that SCFAs play a crucial role in modulating the structure and function of the BBB