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Active DNA demethylation occurs under the catalysis by TET proteins, which sequentially oxidize 5-methylcytosine (5mC) to 5hmC, then to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) [38, 104, 126, 147]

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Analysis of the variant representation in treated vs untreated samples would allow us to determine variants that were depleted in the treated population as compared to the control (Fig

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