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BF skeletal muscle biopsies were obtained from six normally growing late gestation fetal sheep (132 0 days gestation, term gestation is 147 days) and cultured as previously described ( 1 oIGF-1, or DMEM with 10 ng mL 1 oIGF-1

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Commonly used indicators of ROS alteration in intact muscle fibers, such as fluorescent probes and spin traps, have limited specificity to the types of ROS (Powers and Jackson, 2008