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After treatment, cells were washed, tripsinized and resuspended in FACS buffer (PBS with 5% FBS and 1mM EDTA) and analyzed on an LSR Fortessa (BD Biosciences) with plate reader operated on BD FACSDiva software (v9.0)

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Methods Study design and setting Data were obtained from the Netherlands Study of Depression and Anxiety (NESDA), an ongoing naturalistic longitudinal cohort study examining course and consequences of depressive and anxiety disorders