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The cleaved RNA fragments are copied into first-strand cDNA using SuperScript II reverse transcriptase (Invitrogen, 18064014) and random primers

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7880 Neurotrophins, a family of nerve growth factors, such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophic factor 3 (NT-3), and neurotrophic factor 4/5 (NT-4/5), are considered to be endometriosis-associated pain factors

however, the analyses of the effects of these two events are exploratory due to the small sample sizes and consequent limitation in power