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Ultraviolet A light (UVA, 320400 nm) penetrates deeply into the dermis, activating ROS and increases production of matrix metalloproteinases (MMPs) - the enzymes responsible for collagen and elastin breakdown in the dermis (Ritti & Fisher, 2002), as well as causing direct damage to the DNA in skin cells

On day 3 after antibody injection, to boost the inflammatory response, mice were administered an i.p

Temperature control, sterile technique, and injection angle aren't suggestionsthey're the difference between a functional research compound and an expensive vial of denatured protein