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After being taken up, Fe3+ is reduced to Fe2+ in endosomes by the six-transmembrane epithelial antigen of prostate 3 (STEAP3), and then it is transported into the cytosol and free mitochondrial iron pools by solute carrier family 11 member 2(SLC11A2) ( 2+ can cause excessive lipid ROS accumulation through the Fenton reaction and can increase to activate nonheme iron-based arachidonate lipoxygenases (ALOXs) and nicotinamide adenine dinucleotide phosphate oxidases (NOXs), thereby promoting destructive lipid peroxidation ( 2+ is the degradation of hemoglobin mediated by haem oxygenase 1 (HMOX1)

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The absence of a significant change in ruminal pH suggests that YC not only increased microbial productivity but may also have improved ruminal buffering capacity or volatile fatty acid absorption, thereby maintaining a stable and favorable environment for sustained fermentation

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