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Alternatively, 75 mM IAM was added to both cell cultures and cell pellets prior to lysis (B), or only to cell pellets prior to lysis (C), and samples were processed for Western blot analysis as described above

Clinical definitions MS active disease was defined as a clinical attack and/or, at least, one gadolinium enhanced lesion (GEL) in T1-weighted MRI, and/or a new lesion in T2-weighted MRI

EEG during spells was without epileptic discharges, metabolic investigations and QTc were normal, and whole-exome sequencing confirmed biallelic pathogenic TANGO2 variants

Acquisition parameters were: 1.3 s acquisition time, 114 k size of FID, and a relaxation delay (D1) of 15 s

Additionally, ChiA coordinates a magnesium ion via N69/E75 along with their counterparts N69 and E75 from another symmetry-related monomer (Fig