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10.1039/d0lc00981d 74 ZhangL.XueY.MuD.ChenS.WuG.YangZ.et al (2025)

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]