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Monocytes were then purified using negative selection in an LD column magnet separator (Miltenyi Biotech) and monocytes purity was assessed using flow cytometry

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Method Reconstitute the solution in the powder vial with 100ml-500ml of Saline for injection by means of a syringe with an appropriate needle through IV Remove the seal of the vial and wipe closures with disinfectant, then insert the needle of the syringe in the vial through the center of the rubber closure and direct the flow of water toward the glass wall of the vial

Circulating antigen tests and urine reagent strips for diagnosis of active schistosomiasis in endemic areas