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Afterwards, the culture medium was removed and replaced by assay medium (DMEM culture medium supplemented with 2% fetal calf serum, 2 mM L-glutamine, 50 U/ml penicillin, and 50 g/ml streptomycin) containing the test peptides or not (control)

The UCHL1 WT gene was cloned into the ampicillin resistant vector pET-21a containing a C-terminal His 6 tag

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these include significant impairment to ones health or relationships or failure to fulfill major responsibilities at work, school, or home because of substance use

These molecules aid clearance of the damaged tissue but can also induce harm to healthy surrounding tissue ( As shown, muscle injury evoked by exercise is a complex response including multiple cells and multiple molecules (Figure 1)