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AMP-activated protein kinase promotes BRCA metastasis by regulating the phosphorylation of PDHA 30

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Proteins for human CYP1A2, CYP3A4 or EGFR (1 g) were incubated with 10 g of FLAG-PGRMC1 (a.a.44195) treated with or without 50 mol l 1 haemin in 500 l of binding buffer containing 20 mmol l 1 HEPES-NaOH (pH 7.9), 100 mmol l 1 NaCl, 0.2 mmol l 1 EDTA, 10% glycerol and 0.1% NP40 for 60 min at room temperature