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After co-cultured for 12 h at 37 C, the macrophages on the lower surface of inserts were fixed with 4% formaldehyde for 30 min, stained with DAPI, and counted according to the previously established protocol [40]

Por otro lado, algunas opiniones reflejan ciertas limitaciones o consideraciones importantes sobre el uso del producto

This stiffening phenomenon may be linked to increased elastin and collagen content

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However, due to the low solubility of chromium (III) precipitates, only a small amount of toxic ions can pass through the cell membrane (Hu et al