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Based on the nucleotide sequences of the target genes, development of primer sets were designed and used to amplify gene fragments by PCR (Additional file 1: Tables S1S6)

The enzyme that will carry this type of degradation reaction is acetaldehyde dehydrogenase (ALDH)

shp63 #1: GAGTGGAATGACTTCAACTTT, shp63 #2: CATCTGACCTGGCATCTAATT

This product is supplied strictly for research purposes only

With the homozygous pGSH1-URA3 strain, a ~ 50% increase of GSH cellular content was observed in cultures supplemented with 100 g/ml 5-FOA, and this increase was relatively stable compared to that of the previous heterozygous strain (Figure S4A)