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In vitro experiments To plate cells for infection, aliquots of BMDMs were thawed on ice, diluted into 9 ml of DMEM, centrifuged in an Eppendorf 5810 R at 1,200 RPM (290 x G) for 5 min, and the pellet was resuspended in 10 ml BMDM media without antibiotics

Hepatic steatosis is a consequence of lipid acquisition exceeding lipid disposal, i.e., the uptake of fatty acids and de novo lipogenesis surpassing fatty acid oxidation and export

Further analysis by Western blot showed that rhGPx3 mutant with excess amino acid could be recognized by the monoclonal antibody against the hexahistidine tag, but not in the case of the protein treated with factor Xa (Figure 1a and 1b)

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Molecular Profile (CAS 137525-51-0) Storage, Handling, and Reconstitution of the BPC-157 Peptide Store lyophilized powder at -20 C, desiccated, protected from light