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The culture medium was exchanged by an extracellular solution containing (in mM) 140 NaCl, 2.5 KC1, 1 MgCl 2 , 3 CaCl 2 , 25 glucose, and 5 HEPES

The vagus nerve serves as the primary communication highway between gut and brain

doi:10.1152/japplphysiol.00019.2020

It has been shown that the HBD family has a high affinity for LPS in vitro and can directly bind to LPS, thus inhibiting inflammatory response [139, 140]