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Likewise, a circRNA derived from ABP gene exons 6 and 7 was discovered in rat testes, where the acceptor splice site of exon 6 is connected to the donor splice site of exon 7 [484], This implies that the creation of mRNA isoforms that omit those same exons in the testes may be connected to or impacted by the generation of circRNAs with distinct exon combinations [484]

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Then, all animals were euthanized by bleeding after transection of the abdominal vena cava under anesthesia and the liver, heart, soleus muscle, gastrocnemius muscle, tibialis anterior muscle, extensor digitorum longus muscle, epididymal white adipose tissue, and brown adipose tissue were harvested and immersed in RNAlater solution (Qiagen, Venlo, Netherlands) for qPCR analysis