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All animal experiments were performed in accordance with the guidelines of the Ethics Committee of the Fifth Affiliated Hospital of Zhengzhou University (KY2023038)

Additionally, other studies have indicated that madecassoside can significantly inhibit the migration ability of KFs by suppressing the activity of the p38 mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways, reducing the phosphorylation level of cofilin, and decreasing the formation of F-actin filaments

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(227) demonstrated that increased ROS in the cytoplasm activate transmembrane protein 16F (TMEM16F), leading to outward chloride ion flux and disruption of membrane phospholipids